| Conference | ||||
| Date | Home | H | A | Away |
| 05/05/2001 | Boston United (P) | 5 | 3 | Hereford United |
| 05/05/2001 | Chester City (R) | 1 | 2 | Rushden & Diamonds |
| 05/05/2001 | Doncaster Rovers | 1 | 2 | Telford United |
| 05/05/2001 | Dover Athletic | 1 | 0 | Stevenage Borough |
| 05/05/2001 | Hednesford Town | 1 | 3 | Hayes |
| 05/05/2001 | Kettering Town | 2 | 0 | Woking |
| 05/05/2001 | Kingstonian | 0 | 1 | Forest Green Rovers |
| 05/05/2001 | Morecambe | 1 | 2 | Leigh RMI (P) |
| 05/05/2001 | Northwich Victoria | 2 | 2 | Nuneaton Borough |
| 05/05/2001 | Southport | 0 | 1 | Dagenham & Redbridge (P) |
| 05/05/2001 | Yeovil Town | 0 | 1 | Scarborough |
| 03/05/2001 | Morecambe | 2 | 3 | Dagenham & Redbridge (P) |
| 01/05/2001 | Chester City (R) | 3 | 2 | Scarborough |
| 01/05/2001 | Forest Green Rovers | 0 | 0 | Nuneaton Borough |
| 01/05/2001 | Telford United | 2 | 0 | Morecambe |
| 01/05/2001 | Yeovil Town | 2 | 3 | Hereford United |
| 30/04/2001 | Stevenage Borough | 1 | 3 | Southport |
| 28/04/2001 | Dagenham & Redbridge (P) | 1 | 0 | Northwich Victoria |
| 28/04/2001 | Forest Green Rovers | 2 | 0 | Southport |
| 28/04/2001 | Hayes | 1 | 1 | Boston United (P) |
| 28/04/2001 | Hereford United | 2 | 2 | Morecambe |
| 28/04/2001 | Leigh RMI (P) | 2 | 1 | Kingstonian |
| 28/04/2001 | Nuneaton Borough | 1 | 1 | Kettering Town |
| 28/04/2001 | Rushden & Diamonds | 2 | 1 | Dover Athletic |
| 28/04/2001 | Scarborough | 0 | 0 | Hednesford Town |
| 28/04/2001 | Stevenage Borough | 0 | 0 | Doncaster Rovers |
| 28/04/2001 | Telford United | 1 | 2 | Yeovil Town |
| 28/04/2001 | Woking | 1 | 0 | Chester City (R) |
| 26/04/2001 | Chester City (R) | 0 | 0 | Kingstonian |
| 26/04/2001 | Leigh RMI (P) | 1 | 0 | Morecambe |
| 25/04/2001 | Forest Green Rovers | 0 | 0 | Woking |
| 24/04/2001 | Kettering Town | 4 | 0 | Chester City (R) |
| 24/04/2001 | Kingstonian | 0 | 0 | Dover Athletic |
| 24/04/2001 | Leigh RMI (P) | 2 | 3 | Yeovil Town |
| 24/04/2001 | Nuneaton Borough | 3 | 1 | Northwich Victoria |
| 24/04/2001 | Rushden & Diamonds | 3 | 0 | Telford United |
| 23/04/2001 | Hednesford Town | 2 | 4 | Boston United (P) |
| 23/04/2001 | Stevenage Borough | 1 | 1 | Morecambe |
| 21/04/2001 | Boston United (P) | 2 | 1 | Kingstonian |
| 21/04/2001 | Chester City (R) | 1 | 1 | Dagenham & Redbridge (P) |
| 21/04/2001 | Doncaster Rovers | 4 | 0 | Leigh RMI (P) |
| 21/04/2001 | Hereford United | 1 | 1 | Scarborough |
| 21/04/2001 | Kettering Town | 1 | 2 | Stevenage Borough |
| 21/04/2001 | Morecambe | 0 | 2 | Forest Green Rovers |
| 21/04/2001 | Nuneaton Borough | 1 | 2 | Dover Athletic |
| 21/04/2001 | Southport | 2 | 0 | Hednesford Town |
| 21/04/2001 | Woking | 1 | 1 | Northwich Victoria |
| 21/04/2001 | Yeovil Town | 0 | 0 | Rushden & Diamonds |
| 19/04/2001 | Hednesford Town | 1 | 1 | Forest Green Rovers |
| 18/04/2001 | Hereford United | 0 | 0 | Kingstonian |
| 18/04/2001 | Leigh RMI (P) | 0 | 1 | Chester City (R) |
| 18/04/2001 | Morecambe | 2 | 1 | Doncaster Rovers |
| 18/04/2001 | Telford United | 2 | 2 | Stevenage Borough |
| 16/04/2001 | Dagenham & Redbridge (P) | 2 | 0 | Yeovil Town |
| 16/04/2001 | Dover Athletic | 0 | 1 | Southport |
| 16/04/2001 | Forest Green Rovers | 1 | 1 | Hereford United |
| 16/04/2001 | Hayes | 1 | 1 | Morecambe |
| 16/04/2001 | Hednesford Town | 0 | 3 | Nuneaton Borough |
| 16/04/2001 | Kingstonian | 0 | 1 | Telford United |
| 16/04/2001 | Leigh RMI (P) | 2 | 0 | Woking |
| 16/04/2001 | Northwich Victoria | 1 | 2 | Kettering Town |
| 16/04/2001 | Rushden & Diamonds | 0 | 0 | Doncaster Rovers |
| 16/04/2001 | Scarborough | 2 | 2 | Boston United (P) |
| 16/04/2001 | Stevenage Borough | 1 | 2 | Chester City (R) |
| 14/04/2001 | Chester City (R) | 0 | 1 | Forest Green Rovers |
| 14/04/2001 | Doncaster Rovers | 0 | 0 | Hayes |
| 14/04/2001 | Hereford United | 3 | 1 | Rushden & Diamonds |
| 14/04/2001 | Kettering Town | 2 | 0 | Hednesford Town |
| 14/04/2001 | Morecambe | 1 | 2 | Dover Athletic |
| 14/04/2001 | Nuneaton Borough | 1 | 2 | Scarborough |
| 14/04/2001 | Southport | 2 | 2 | Kingstonian |
| 14/04/2001 | Telford United | 2 | 3 | Northwich Victoria |
| 14/04/2001 | Woking | 4 | 4 | Dagenham & Redbridge (P) |
| 14/04/2001 | Yeovil Town | 6 | 1 | Leigh RMI (P) |
| 12/04/2001 | Nuneaton Borough | 5 | 1 | Morecambe |
| 11/04/2001 | Boston United (P) | 5 | 1 | Dagenham & Redbridge (P) |
| 10/04/2001 | Doncaster Rovers | 2 | 0 | Yeovil Town |
| 10/04/2001 | Dover Athletic | 1 | 0 | Hereford United |
| 10/04/2001 | Leigh RMI (P) | 1 | 4 | Stevenage Borough |
| 10/04/2001 | Northwich Victoria | 2 | 1 | Kingstonian |
| 10/04/2001 | Scarborough | 1 | 1 | Telford United |
| 10/04/2001 | Woking | 1 | 2 | Southport |
| 07/04/2001 | Dagenham & Redbridge (P) | 0 | 0 | Telford United |
| 07/04/2001 | Hayes | 1 | 0 | Southport |
| 07/04/2001 | Kingstonian | 3 | 4 | Yeovil Town |
| 07/04/2001 | Rushden & Diamonds | 4 | 1 | Morecambe |
| 07/04/2001 | Scarborough | 0 | 1 | Kettering Town |
| 07/04/2001 | Stevenage Borough | 1 | 1 | Nuneaton Borough |
| 03/04/2001 | Dover Athletic | 1 | 1 | Chester City (R) |
| 03/04/2001 | Hereford United | 0 | 1 | Woking |
| 03/04/2001 | Kingstonian | 2 | 2 | Scarborough |
| 03/04/2001 | Leigh RMI (P) | 1 | 0 | Kettering Town |
| 02/04/2001 | Stevenage Borough | 4 | 1 | Hednesford Town |
| 31/03/2001 | Doncaster Rovers | 0 | 0 | Kettering Town |
| 31/03/2001 | Dover Athletic | 1 | 1 | Yeovil Town |
| 31/03/2001 | Hayes | 4 | 1 | Dagenham & Redbridge (P) |
| 31/03/2001 | Morecambe | 1 | 2 | Stevenage Borough |
| 31/03/2001 | Nuneaton Borough | 2 | 1 | Kingstonian |
| 31/03/2001 | Rushden & Diamonds | 1 | 1 | Leigh RMI (P) |
| 31/03/2001 | Southport | 1 | 1 | Northwich Victoria |
| 31/03/2001 | Telford United | 3 | 2 | Boston United (P) |
| 28/03/2001 | Forest Green Rovers | 3 | 1 | Leigh RMI (P) |
| 27/03/2001 | Dagenham & Redbridge (P) | 2 | 1 | Doncaster Rovers |
| 27/03/2001 | Southport | 1 | 1 | Hereford United |
| 27/03/2001 | Telford United | 3 | 0 | Chester City (R) |
| 26/03/2001 | Hednesford Town | 0 | 0 | Dover Athletic |
| 26/03/2001 | Stevenage Borough | 0 | 3 | Woking |
| 25/03/2001 | Boston United (P) | 1 | 0 | Southport |
| 25/03/2001 | Dagenham & Redbridge (P) | 3 | 2 | Morecambe |
| 25/03/2001 | Northwich Victoria | 0 | 0 | Rushden & Diamonds |
| 25/03/2001 | Scarborough | 1 | 0 | Forest Green Rovers |
| 24/03/2001 | Hednesford Town | 2 | 4 | Doncaster Rovers |
| 24/03/2001 | Yeovil Town | 3 | 0 | Hayes |
| 23/03/2001 | Kettering Town | 0 | 1 | Telford United |
| 23/03/2001 | Leigh RMI (P) | 2 | 1 | Hereford United |
| 23/03/2001 | Nuneaton Borough | 1 | 2 | Chester City (R) |
| 20/03/2001 | Dagenham & Redbridge (P) | 3 | 0 | Stevenage Borough |
| 17/03/2001 | Chester City (R) | 0 | 1 | Hednesford Town |
| 17/03/2001 | Doncaster Rovers | 4 | 2 | Boston United (P) |
| 17/03/2001 | Dover Athletic | 1 | 2 | Leigh RMI (P) |
| 17/03/2001 | Forest Green Rovers | 3 | 2 | Kettering Town |
| 17/03/2001 | Hayes | 2 | 2 | Northwich Victoria |
| 17/03/2001 | Hereford United | 0 | 1 | Dagenham & Redbridge (P) |
| 17/03/2001 | Morecambe | 0 | 0 | Yeovil Town |
| 17/03/2001 | Rushden & Diamonds | 2 | 1 | Kingstonian |
| 17/03/2001 | Southport | 1 | 2 | Nuneaton Borough |
| 17/03/2001 | Woking | 1 | 1 | Scarborough |
| 13/03/2001 | Hayes | 1 | 0 | Forest Green Rovers |
| 13/03/2001 | Kettering Town | 0 | 2 | Rushden & Diamonds |
| 13/03/2001 | Southport | 1 | 0 | Chester City (R) |
| 13/03/2001 | Telford United | 0 | 1 | Kingstonian |
| 13/03/2001 | Woking | 3 | 1 | Morecambe |
| 13/03/2001 | Yeovil Town | 0 | 0 | Nuneaton Borough |
| 12/03/2001 | Stevenage Borough | 2 | 1 | Hereford United |
| 10/03/2001 | Boston United (P) | 1 | 1 | Rushden & Diamonds |
| 10/03/2001 | Kettering Town | 2 | 1 | Yeovil Town |
| 10/03/2001 | Kingstonian | 1 | 6 | Morecambe |
| 10/03/2001 | Leigh RMI (P) | 4 | 0 | Hayes |
| 10/03/2001 | Northwich Victoria | 2 | 0 | Dover Athletic |
| 06/03/2001 | Dagenham & Redbridge (P) | 1 | 0 | Scarborough |
| 06/03/2001 | Morecambe | 4 | 0 | Northwich Victoria |
| 03/03/2001 | Dagenham & Redbridge (P) | 3 | 1 | Forest Green Rovers |
| 03/03/2001 | Dover Athletic | 1 | 3 | Telford United |
| 03/03/2001 | Hayes | 1 | 3 | Chester City (R) |
| 03/03/2001 | Kingstonian | 1 | 0 | Hednesford Town |
| 03/03/2001 | Leigh RMI (P) | 6 | 2 | Nuneaton Borough |
| 03/03/2001 | Morecambe | 2 | 0 | Boston United (P) |
| 03/03/2001 | Northwich Victoria | 3 | 0 | Scarborough |
| 03/03/2001 | Rushden & Diamonds | 2 | 0 | Woking |
| 03/03/2001 | Southport | 2 | 3 | Kettering Town |
| 03/03/2001 | Yeovil Town | 1 | 1 | Stevenage Borough |
| 27/02/2001 | Chester City (R) | 2 | 2 | Boston United (P) |
| 27/02/2001 | Hayes | 1 | 1 | Kingstonian |
| 27/02/2001 | Woking | 3 | 0 | Telford United |
| 24/02/2001 | Boston United (P) | 1 | 1 | Northwich Victoria |
| 24/02/2001 | Hednesford Town | 0 | 2 | Dagenham & Redbridge (P) |
| 24/02/2001 | Nuneaton Borough | 1 | 1 | Hayes |
| 24/02/2001 | Scarborough | 2 | 0 | Dover Athletic |
| 24/02/2001 | Woking | 1 | 1 | Kettering Town |
| 20/02/2001 | Chester City (R) | 1 | 0 | Telford United |
| 20/02/2001 | Kingstonian | 0 | 1 | Kettering Town |
| 20/02/2001 | Rushden & Diamonds | 2 | 1 | Northwich Victoria |
| 20/02/2001 | Yeovil Town | 1 | 3 | Dagenham & Redbridge (P) |
| 17/02/2001 | Dagenham & Redbridge (P) | 5 | 1 | Kettering Town |
| 17/02/2001 | Doncaster Rovers | 1 | 1 | Dover Athletic |
| 17/02/2001 | Hayes | 1 | 2 | Woking |
| 17/02/2001 | Hereford United | 1 | 1 | Nuneaton Borough |
| 17/02/2001 | Kingstonian | 0 | 2 | Stevenage Borough |
| 17/02/2001 | Morecambe | 0 | 0 | Hednesford Town |
| 17/02/2001 | Northwich Victoria | 1 | 1 | Chester City (R) |
| 17/02/2001 | Rushden & Diamonds | 1 | 0 | Scarborough |
| 17/02/2001 | Southport | 1 | 1 | Forest Green Rovers |
| 17/02/2001 | Yeovil Town | 2 | 1 | Boston United (P) |
| 13/02/2001 | Telford United | 2 | 1 | Hednesford Town |
| 10/02/2001 | Boston United (P) | 0 | 1 | Leigh RMI (P) |
| 10/02/2001 | Chester City (R) | 2 | 1 | Yeovil Town |
| 10/02/2001 | Doncaster Rovers | 0 | 2 | Kingstonian |
| 10/02/2001 | Forest Green Rovers | 0 | 0 | Rushden & Diamonds |
| 10/02/2001 | Hednesford Town | 0 | 3 | Hereford United |
| 10/02/2001 | Kettering Town | 1 | 5 | Morecambe |
| 10/02/2001 | Nuneaton Borough | 2 | 0 | Dagenham & Redbridge (P) |
| 10/02/2001 | Scarborough | 2 | 0 | Hayes |
| 10/02/2001 | Stevenage Borough | 3 | 1 | Northwich Victoria |
| 10/02/2001 | Telford United | 2 | 3 | Southport |
| 10/02/2001 | Woking | 4 | 1 | Dover Athletic |
| 06/02/2001 | Telford United | 1 | 0 | Doncaster Rovers |
| 03/02/2001 | Northwich Victoria | 2 | 2 | Hednesford Town |
| 03/02/2001 | Nuneaton Borough | 1 | 0 | Doncaster Rovers |
| 29/01/2001 | Hednesford Town | 1 | 2 | Yeovil Town |
| 27/01/2001 | Chester City (R) | 1 | 0 | Morecambe |
| 27/01/2001 | Dover Athletic | 1 | 1 | Doncaster Rovers |
| 27/01/2001 | Forest Green Rovers | 1 | 0 | Northwich Victoria |
| 27/01/2001 | Hednesford Town | 1 | 2 | Leigh RMI (P) |
| 27/01/2001 | Kettering Town | 0 | 2 | Hayes |
| 27/01/2001 | Nuneaton Borough | 1 | 1 | Woking |
| 27/01/2001 | Scarborough | 1 | 1 | Southport |
| 27/01/2001 | Stevenage Borough | 0 | 2 | Rushden & Diamonds |
| 27/01/2001 | Telford United | 1 | 0 | Hereford United |
| 20/01/2001 | Boston United (P) | 0 | 0 | Woking |
| 20/01/2001 | Doncaster Rovers | 3 | 0 | Forest Green Rovers |
| 20/01/2001 | Hereford United | 3 | 2 | Hayes |
| 20/01/2001 | Telford United | 2 | 1 | Nuneaton Borough |
| 20/01/2001 | Yeovil Town | 0 | 1 | Southport |
| 06/01/2001 | Boston United (P) | 3 | 3 | Stevenage Borough |
| 06/01/2001 | Forest Green Rovers | 1 | 1 | Telford United |
| 06/01/2001 | Hayes | 1 | 1 | Hednesford Town |
| 06/01/2001 | Kettering Town | 0 | 2 | Dover Athletic |
| 06/01/2001 | Northwich Victoria | 1 | 0 | Hereford United |
| 06/01/2001 | Scarborough | 1 | 1 | Leigh RMI (P) |
| 06/01/2001 | Southport | 1 | 3 | Rushden & Diamonds |
| 06/01/2001 | Woking | 1 | 1 | Doncaster Rovers |
| 01/01/2001 | Boston United (P) | 4 | 3 | Kettering Town |
| 01/01/2001 | Doncaster Rovers | 0 | 2 | Scarborough |
| 01/01/2001 | Dover Athletic | 3 | 1 | Dagenham & Redbridge (P) |
| 01/01/2001 | Hereford United | 2 | 0 | Chester City (R) |
| 01/01/2001 | Kingstonian | 0 | 3 | Woking |
| 01/01/2001 | Leigh RMI (P) | 3 | 0 | Northwich Victoria |
| 01/01/2001 | Morecambe | 1 | 3 | Southport |
| 01/01/2001 | Rushden & Diamonds | 2 | 1 | Nuneaton Borough |
| 01/01/2001 | Stevenage Borough | 3 | 3 | Hayes |
| 01/01/2001 | Yeovil Town | 2 | 0 | Forest Green Rovers |
| 30/12/2000 | Dagenham & Redbridge (P) | 2 | 1 | Leigh RMI (P) |
| 30/12/2000 | Forest Green Rovers | 0 | 3 | Boston United (P) |
| 26/12/2000 | Chester City (R) | 2 | 1 | Hereford United |
| 26/12/2000 | Dagenham & Redbridge (P) | 1 | 1 | Dover Athletic |
| 26/12/2000 | Forest Green Rovers | 0 | 1 | Yeovil Town |
| 26/12/2000 | Hayes | 0 | 1 | Stevenage Borough |
| 26/12/2000 | Hednesford Town | 1 | 1 | Telford United |
| 26/12/2000 | Kettering Town | 2 | 2 | Boston United (P) |
| 26/12/2000 | Northwich Victoria | 1 | 1 | Leigh RMI (P) |
| 26/12/2000 | Nuneaton Borough | 1 | 1 | Rushden & Diamonds |
| 26/12/2000 | Scarborough | 3 | 1 | Doncaster Rovers |
| 26/12/2000 | Southport | 1 | 2 | Morecambe |
| 26/12/2000 | Woking | 0 | 0 | Kingstonian |
| 23/12/2000 | Chester City (R) | 0 | 0 | Hayes |
| 22/12/2000 | Southport | 2 | 2 | Stevenage Borough |
| 16/12/2000 | Boston United (P) | 4 | 1 | Nuneaton Borough |
| 16/12/2000 | Doncaster Rovers | 1 | 0 | Chester City (R) |
| 16/12/2000 | Dover Athletic | 4 | 1 | Hayes |
| 16/12/2000 | Hereford United | 0 | 0 | Kettering Town |
| 16/12/2000 | Kingstonian | 2 | 3 | Dagenham & Redbridge (P) |
| 16/12/2000 | Leigh RMI (P) | 2 | 2 | Southport |
| 16/12/2000 | Morecambe | 4 | 4 | Scarborough |
| 16/12/2000 | Rushden & Diamonds | 5 | 1 | Hednesford Town |
| 16/12/2000 | Stevenage Borough | 3 | 1 | Forest Green Rovers |
| 16/12/2000 | Telford United | 3 | 1 | Woking |
| 16/12/2000 | Yeovil Town | 1 | 0 | Northwich Victoria |
| 09/12/2000 | Hayes | 0 | 3 | Rushden & Diamonds |
| 09/12/2000 | Hereford United | 0 | 1 | Doncaster Rovers |
| 09/12/2000 | Woking | 1 | 1 | Hednesford Town |
| 02/12/2000 | Boston United (P) | 0 | 1 | Hayes |
| 02/12/2000 | Chester City (R) | 3 | 3 | Woking |
| 02/12/2000 | Doncaster Rovers | 0 | 0 | Stevenage Borough |
| 02/12/2000 | Dover Athletic | 4 | 1 | Rushden & Diamonds |
| 02/12/2000 | Hednesford Town | 0 | 1 | Scarborough |
| 02/12/2000 | Kettering Town | 1 | 2 | Nuneaton Borough |
| 02/12/2000 | Kingstonian | 0 | 2 | Leigh RMI (P) |
| 02/12/2000 | Morecambe | 1 | 1 | Hereford United |
| 02/12/2000 | Northwich Victoria | 3 | 0 | Dagenham & Redbridge (P) |
| 02/12/2000 | Yeovil Town | 2 | 0 | Telford United |
| 28/11/2000 | Southport | 1 | 0 | Doncaster Rovers |
| 25/11/2000 | Dagenham & Redbridge (P) | 0 | 1 | Southport |
| 25/11/2000 | Forest Green Rovers | 2 | 1 | Dover Athletic |
| 25/11/2000 | Hereford United | 1 | 1 | Boston United (P) |
| 25/11/2000 | Scarborough | 2 | 2 | Yeovil Town |
| 25/11/2000 | Stevenage Borough | 2 | 5 | Kingstonian |
| 18/11/2000 | Dover Athletic | 0 | 0 | Boston United (P) |
| 18/11/2000 | Scarborough | 2 | 2 | Stevenage Borough |
| 11/11/2000 | Boston United (P) | 2 | 2 | Scarborough |
| 11/11/2000 | Chester City (R) | 1 | 1 | Stevenage Borough |
| 11/11/2000 | Doncaster Rovers | 3 | 2 | Rushden & Diamonds |
| 11/11/2000 | Hereford United | 3 | 1 | Forest Green Rovers |
| 11/11/2000 | Kettering Town | 2 | 3 | Northwich Victoria |
| 11/11/2000 | Morecambe | 4 | 0 | Hayes |
| 11/11/2000 | Nuneaton Borough | 5 | 1 | Hednesford Town |
| 11/11/2000 | Southport | 2 | 1 | Dover Athletic |
| 11/11/2000 | Woking | 1 | 1 | Leigh RMI (P) |
| 04/11/2000 | Dagenham & Redbridge (P) | 1 | 1 | Chester City (R) |
| 04/11/2000 | Dover Athletic | 2 | 1 | Nuneaton Borough |
| 04/11/2000 | Forest Green Rovers | 0 | 0 | Morecambe |
| 04/11/2000 | Hayes | 0 | 1 | Telford United |
| 04/11/2000 | Hednesford Town | 0 | 1 | Southport |
| 04/11/2000 | Kingstonian | 0 | 0 | Boston United (P) |
| 04/11/2000 | Leigh RMI (P) | 0 | 1 | Doncaster Rovers |
| 04/11/2000 | Northwich Victoria | 4 | 0 | Woking |
| 04/11/2000 | Rushden & Diamonds | 1 | 2 | Yeovil Town |
| 04/11/2000 | Scarborough | 2 | 4 | Hereford United |
| 04/11/2000 | Stevenage Borough | 2 | 0 | Kettering Town |
| 31/10/2000 | Northwich Victoria | 0 | 0 | Forest Green Rovers |
| 31/10/2000 | Rushden & Diamonds | 4 | 0 | Hayes |
| 21/10/2000 | Boston United (P) | 3 | 4 | Hednesford Town |
| 21/10/2000 | Chester City (R) | 1 | 1 | Leigh RMI (P) |
| 21/10/2000 | Hereford United | 4 | 2 | Dover Athletic |
| 21/10/2000 | Kettering Town | 1 | 1 | Scarborough |
| 21/10/2000 | Morecambe | 2 | 1 | Rushden & Diamonds |
| 21/10/2000 | Nuneaton Borough | 0 | 3 | Stevenage Borough |
| 21/10/2000 | Southport | 2 | 0 | Hayes |
| 21/10/2000 | Telford United | 0 | 1 | Dagenham & Redbridge (P) |
| 21/10/2000 | Woking | 2 | 0 | Forest Green Rovers |
| 21/10/2000 | Yeovil Town | 3 | 1 | Kingstonian |
| 20/10/2000 | Doncaster Rovers | 0 | 2 | Northwich Victoria |
| 14/10/2000 | Dagenham & Redbridge (P) | 1 | 2 | Woking |
| 14/10/2000 | Dover Athletic | 2 | 2 | Morecambe |
| 14/10/2000 | Forest Green Rovers | 1 | 1 | Chester City (R) |
| 14/10/2000 | Hayes | 0 | 3 | Doncaster Rovers |
| 14/10/2000 | Hednesford Town | 1 | 2 | Kettering Town |
| 14/10/2000 | Kingstonian | 3 | 1 | Southport |
| 14/10/2000 | Leigh RMI (P) | 1 | 1 | Telford United |
| 14/10/2000 | Northwich Victoria | 1 | 2 | Yeovil Town |
| 14/10/2000 | Rushden & Diamonds | 1 | 0 | Hereford United |
| 14/10/2000 | Scarborough | 0 | 0 | Nuneaton Borough |
| 14/10/2000 | Stevenage Borough | 3 | 2 | Boston United (P) |
| 08/10/2000 | Chester City (R) | 2 | 1 | Kettering Town |
| 08/10/2000 | Dover Athletic | 3 | 0 | Northwich Victoria |
| 08/10/2000 | Forest Green Rovers | 0 | 2 | Hednesford Town |
| 08/10/2000 | Hereford United | 2 | 2 | Yeovil Town |
| 08/10/2000 | Morecambe | 3 | 2 | Kingstonian |
| 08/10/2000 | Rushden & Diamonds | 0 | 0 | Boston United (P) |
| 07/10/2000 | Hayes | 1 | 2 | Leigh RMI (P) |
| 06/10/2000 | Doncaster Rovers | 1 | 0 | Dagenham & Redbridge (P) |
| 06/10/2000 | Telford United | 1 | 0 | Scarborough |
| 06/10/2000 | Woking | 0 | 2 | Nuneaton Borough |
| 04/10/2000 | Boston United (P) | 2 | 1 | Telford United |
| 03/10/2000 | Dagenham & Redbridge (P) | 2 | 0 | Hayes |
| 03/10/2000 | Kettering Town | 0 | 0 | Doncaster Rovers |
| 03/10/2000 | Kingstonian | 0 | 3 | Hereford United |
| 03/10/2000 | Leigh RMI (P) | 1 | 0 | Rushden & Diamonds |
| 03/10/2000 | Northwich Victoria | 0 | 2 | Southport |
| 03/10/2000 | Nuneaton Borough | 2 | 0 | Forest Green Rovers |
| 03/10/2000 | Scarborough | 0 | 2 | Chester City (R) |
| 03/10/2000 | Yeovil Town | 4 | 0 | Dover Athletic |
| 02/10/2000 | Hednesford Town | 1 | 2 | Woking |
| 30/09/2000 | Boston United (P) | 3 | 1 | Doncaster Rovers |
| 30/09/2000 | Dagenham & Redbridge (P) | 2 | 1 | Hereford United |
| 30/09/2000 | Hednesford Town | 0 | 0 | Chester City (R) |
| 30/09/2000 | Kettering Town | 1 | 3 | Forest Green Rovers |
| 30/09/2000 | Kingstonian | 2 | 4 | Rushden & Diamonds |
| 30/09/2000 | Leigh RMI (P) | 2 | 1 | Dover Athletic |
| 30/09/2000 | Northwich Victoria | 3 | 4 | Hayes |
| 30/09/2000 | Nuneaton Borough | 1 | 2 | Southport |
| 30/09/2000 | Scarborough | 3 | 2 | Woking |
| 30/09/2000 | Stevenage Borough | 5 | 3 | Telford United |
| 30/09/2000 | Yeovil Town | 3 | 2 | Morecambe |
| 27/09/2000 | Forest Green Rovers | 2 | 3 | Scarborough |
| 26/09/2000 | Chester City (R) | 4 | 0 | Nuneaton Borough |
| 26/09/2000 | Doncaster Rovers | 3 | 1 | Hednesford Town |
| 26/09/2000 | Dover Athletic | 1 | 3 | Kingstonian |
| 26/09/2000 | Hayes | 2 | 3 | Yeovil Town |
| 26/09/2000 | Hereford United | 1 | 1 | Leigh RMI (P) |
| 26/09/2000 | Rushden & Diamonds | 2 | 1 | Dagenham & Redbridge (P) |
| 26/09/2000 | Southport | 3 | 1 | Boston United (P) |
| 26/09/2000 | Telford United | 2 | 1 | Kettering Town |
| 26/09/2000 | Woking | 1 | 1 | Stevenage Borough |
| 23/09/2000 | Dagenham & Redbridge (P) | 6 | 1 | Hednesford Town |
| 23/09/2000 | Dover Athletic | 0 | 2 | Scarborough |
| 23/09/2000 | Hayes | 0 | 0 | Nuneaton Borough |
| 23/09/2000 | Hereford United | 1 | 1 | Stevenage Borough |
| 23/09/2000 | Kingstonian | 1 | 3 | Chester City (R) |
| 23/09/2000 | Leigh RMI (P) | 1 | 1 | Forest Green Rovers |
| 23/09/2000 | Morecambe | 0 | 0 | Telford United |
| 23/09/2000 | Northwich Victoria | 0 | 3 | Boston United (P) |
| 23/09/2000 | Rushden & Diamonds | 1 | 1 | Kettering Town |
| 23/09/2000 | Southport | 0 | 1 | Woking |
| 23/09/2000 | Yeovil Town | 2 | 0 | Doncaster Rovers |
| 16/09/2000 | Boston United (P) | 2 | 1 | Morecambe |
| 16/09/2000 | Doncaster Rovers | 2 | 1 | Hereford United |
| 16/09/2000 | Forest Green Rovers | 4 | 4 | Dagenham & Redbridge (P) |
| 16/09/2000 | Hednesford Town | 3 | 2 | Kingstonian |
| 16/09/2000 | Kettering Town | 1 | 1 | Southport |
| 16/09/2000 | Nuneaton Borough | 2 | 1 | Leigh RMI (P) |
| 16/09/2000 | Scarborough | 4 | 0 | Northwich Victoria |
| 16/09/2000 | Stevenage Borough | 0 | 0 | Yeovil Town |
| 16/09/2000 | Telford United | 0 | 2 | Dover Athletic |
| 16/09/2000 | Woking | 1 | 4 | Rushden & Diamonds |
| 13/09/2000 | Boston United (P) | 4 | 1 | Yeovil Town |
| 13/09/2000 | Forest Green Rovers | 3 | 1 | Kingstonian |
| 12/09/2000 | Chester City (R) | 1 | 1 | Northwich Victoria |
| 12/09/2000 | Doncaster Rovers | 1 | 0 | Southport |
| 12/09/2000 | Kettering Town | 0 | 0 | Dagenham & Redbridge (P) |
| 12/09/2000 | Nuneaton Borough | 1 | 2 | Hereford United |
| 12/09/2000 | Scarborough | 0 | 3 | Rushden & Diamonds |
| 12/09/2000 | Telford United | 2 | 1 | Leigh RMI (P) |
| 12/09/2000 | Woking | 1 | 2 | Hayes |
| 11/09/2000 | Hednesford Town | 0 | 0 | Morecambe |
| 11/09/2000 | Stevenage Borough | 1 | 1 | Dover Athletic |
| 09/09/2000 | Dagenham & Redbridge (P) | 1 | 1 | Nuneaton Borough |
| 09/09/2000 | Dover Athletic | 0 | 0 | Woking |
| 09/09/2000 | Hayes | 0 | 1 | Scarborough |
| 09/09/2000 | Hereford United | 1 | 1 | Hednesford Town |
| 09/09/2000 | Kingstonian | 1 | 1 | Doncaster Rovers |
| 09/09/2000 | Leigh RMI (P) | 2 | 2 | Boston United (P) |
| 09/09/2000 | Morecambe | 0 | 2 | Kettering Town |
| 09/09/2000 | Northwich Victoria | 3 | 2 | Stevenage Borough |
| 09/09/2000 | Rushden & Diamonds | 0 | 0 | Forest Green Rovers |
| 09/09/2000 | Southport | 3 | 0 | Telford United |
| 09/09/2000 | Yeovil Town | 2 | 1 | Chester City (R) |
| 05/09/2000 | Dagenham & Redbridge (P) | 2 | 1 | Boston United (P) |
| 05/09/2000 | Dover Athletic | 1 | 2 | Forest Green Rovers |
| 05/09/2000 | Hayes | 2 | 1 | Kettering Town |
| 05/09/2000 | Hereford United | 2 | 0 | Telford United |
| 05/09/2000 | Kingstonian | 2 | 2 | Nuneaton Borough |
| 05/09/2000 | Leigh RMI (P) | 2 | 2 | Hednesford Town |
| 05/09/2000 | Morecambe | 0 | 2 | Chester City (R) |
| 05/09/2000 | Northwich Victoria | 1 | 1 | Doncaster Rovers |
| 05/09/2000 | Rushden & Diamonds | 2 | 2 | Stevenage Borough |
| 05/09/2000 | Southport | 3 | 1 | Scarborough |
| 05/09/2000 | Yeovil Town | 1 | 0 | Woking |
| 02/09/2000 | Boston United (P) | 1 | 2 | Dover Athletic |
| 02/09/2000 | Chester City (R) | 0 | 1 | Southport |
| 02/09/2000 | Doncaster Rovers | 1 | 0 | Morecambe |
| 02/09/2000 | Forest Green Rovers | 1 | 2 | Hayes |
| 02/09/2000 | Hednesford Town | 7 | 1 | Northwich Victoria |
| 02/09/2000 | Kettering Town | 3 | 1 | Kingstonian |
| 02/09/2000 | Nuneaton Borough | 0 | 2 | Yeovil Town |
| 02/09/2000 | Scarborough | 0 | 1 | Dagenham & Redbridge (P) |
| 02/09/2000 | Stevenage Borough | 3 | 0 | Leigh RMI (P) |
| 02/09/2000 | Telford United | 1 | 2 | Rushden & Diamonds |
| 02/09/2000 | Woking | 0 | 3 | Hereford United |
| 30/08/2000 | Boston United (P) | 0 | 0 | Chester City (R) |
| 28/08/2000 | Doncaster Rovers | 0 | 1 | Woking |
| 28/08/2000 | Dover Athletic | 1 | 0 | Kettering Town |
| 28/08/2000 | Hereford United | 0 | 0 | Northwich Victoria |
| 28/08/2000 | Kingstonian | 0 | 1 | Hayes |
| 28/08/2000 | Leigh RMI (P) | 2 | 0 | Scarborough |
| 28/08/2000 | Morecambe | 4 | 2 | Nuneaton Borough |
| 28/08/2000 | Rushden & Diamonds | 4 | 0 | Southport |
| 28/08/2000 | Stevenage Borough | 0 | 2 | Dagenham & Redbridge (P) |
| 28/08/2000 | Telford United | 1 | 0 | Forest Green Rovers |
| 28/08/2000 | Yeovil Town | 4 | 2 | Hednesford Town |
| 26/08/2000 | Chester City (R) | 1 | 0 | Dover Athletic |
| 26/08/2000 | Dagenham & Redbridge (P) | 0 | 2 | Rushden & Diamonds |
| 26/08/2000 | Forest Green Rovers | 2 | 2 | Doncaster Rovers |
| 26/08/2000 | Hayes | 0 | 2 | Hereford United |
| 26/08/2000 | Hednesford Town | 1 | 1 | Stevenage Borough |
| 26/08/2000 | Kettering Town | 0 | 1 | Leigh RMI (P) |
| 26/08/2000 | Northwich Victoria | 1 | 0 | Morecambe |
| 26/08/2000 | Nuneaton Borough | 1 | 1 | Telford United |
| 26/08/2000 | Scarborough | 1 | 0 | Kingstonian |
| 26/08/2000 | Southport | 3 | 0 | Yeovil Town |
| 26/08/2000 | Woking | 1 | 1 | Boston United (P) |
| 23/08/2000 | Forest Green Rovers | 2 | 3 | Stevenage Borough |
| 22/08/2000 | Chester City (R) | 3 | 0 | Doncaster Rovers |
| 22/08/2000 | Dagenham & Redbridge (P) | 1 | 2 | Kingstonian |
| 22/08/2000 | Hayes | 3 | 2 | Dover Athletic |
| 22/08/2000 | Kettering Town | 0 | 2 | Hereford United |
| 22/08/2000 | Northwich Victoria | 0 | 1 | Telford United |
| 22/08/2000 | Nuneaton Borough | 3 | 1 | Boston United (P) |
| 22/08/2000 | Scarborough | 2 | 2 | Morecambe |
| 22/08/2000 | Southport | 1 | 2 | Leigh RMI (P) |
| 22/08/2000 | Woking | 2 | 3 | Yeovil Town |
| 21/08/2000 | Hednesford Town | 2 | 3 | Rushden & Diamonds |
| 19/08/2000 | Boston United (P) | 0 | 0 | Forest Green Rovers |
| 19/08/2000 | Doncaster Rovers | 1 | 1 | Nuneaton Borough |
| 19/08/2000 | Dover Athletic | 4 | 0 | Hednesford Town |
| 19/08/2000 | Hereford United | 0 | 0 | Southport |
| 19/08/2000 | Kingstonian | 1 | 0 | Northwich Victoria |
| 19/08/2000 | Leigh RMI (P) | 1 | 2 | Dagenham & Redbridge (P) |
| 19/08/2000 | Morecambe | 3 | 0 | Woking |
| 19/08/2000 | Rushden & Diamonds | 2 | 0 | Chester City (R) |
| 19/08/2000 | Stevenage Borough | 1 | 1 | Scarborough |
| 19/08/2000 | Telford United | 2 | 0 | Hayes |
| 19/08/2000 | Yeovil Town | 2 | 0 | Kettering Town |